|
Foreword
Table of Contents
Acknowledgments
Introduction
Chapter 1. Bacterial Isolation, Identification and Storage
4Principle
4Isolation
4Storage
4Identification
4References
4Appendix
1.1. Important bacterial
characteristics taken into
consideration when coding or
labeling
isolated bacteria
4Appendix 1.2. Scheme for the
identification of bacterial genera
that
are important in aquaculture
Chapter 2.
Disk Diffusion Method
4Principle
4Media
4Inoculum
4Antimicrobial disk
4Control plate
4Incubation
4Reading and measurement of
zones of inhibition
4Interpretation of results
4Rejection criteria
4References
4Appendix 2.1.
Acceptable inhibitory zone diameter (mm) limit of control strains recommended for use in the disk diffusion antimicrobial
sensitivity testing of bacteria isolated from animals
4Appendix 2.2. Zone diameter interpretative standard for veterinary pathogens
Chapter 3. Minimal Inhibitory Concentration (MIC) Test and
Determination of Antimicrobial Resistant Bacteria
4Principle
4Media
4Inoculum
4Control Plate
4Incubation
4Reading the MIC values
4Determination of Antimicrobial
Resistant Bacteria
4Inhibitory concentration at 50%
and 90%
4Determination of antimicrobial
resistant bacteria
References
4Appendix 3.1.
List of culture apparatus, culture media, solvents, control strains,
and antimicrobial agents needed for the minimal inhibitory
concentration (MIC) test
4Appendix 3.2. Preparation of antimicrobial agent stock solution
4Appendix 3.3.
List of solvents and diluents needed for the preparation of
stock solutions of antimicrobial agents
4Appendix 3.4.
Preparation of dilutions of antimicrobial agents for use in the agar
dilution method of minimal inhibitoryconcentration (MIC) test
4Appendix 3.5. Interpretative categories and correlative minimal inhibitory
concentration (MICs) for food-borne pathogens
4Appendix 3.6. Determination of inhibitory concentration at
50% (IC50) and 90 % (IC90) values
4Appendix 3.7.
Table of assigned values to evaluate the breakpoint, which correlates to the minimal inhibitory concentration (MIC)
4Appendix 3.8. Notes on laboratory practice
|
