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SEAFDEC-OIE Seminar/Workshop on Disease Control
in Fish and Shrimp Aquaculture in Southeast Asia-Diagnosis and Husbandry Techniques This seminar/workshop was held at the Conference Room of Sarabia Manor Hotel in Iloilo City, Philippines on 4-6 December 2001. It was co-organized by the Southeast Asian Fisheries Development Center (SEAFDEC) and the Office International des Epizooties (OIE). The meeting was financially supported by the Government of Japan (GOJ) Trust Fund through the Regional Fish Disease Project. Click on the image for larger version
During the seminar, 13 resource persons presented a total of 14 reviews papers, which provided excellent information on the current research on fish and shrimp diseases and control measures.
TABLE 1: Viral
Disease of Shrimp |
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Shrimp Virus WSSV1 |
Available Diagnostic Method PCR8 histopathology including dark field microscopy in situ and dot-blot hybridization |
Limitation acceptability in the region |
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YHV2 |
RT-PCR9 histopathology Western blot |
availability of positive controlavailability of antibody |
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HPV3 |
PCR histopathology Giemsa-strained hepatopancreas impression smears |
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MBV4 |
PCR malachite green-strained hepatopancreas impression smears |
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IHHNV5 |
PCR in situ hybridization dot-blot hybridization histopathology |
acceptability in the region |
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TSV6 |
RT-PCR in situ hybridization histopathologyantibody-based method |
availability of positive controlacceptability in the regionavailability of antibody |
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BP7 |
wet mounts Histopathology |
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1
White spot syndrome virus; 2 Yellow Head virus; 3
Hepatopancreatic parvovirus 4 Monodon baculovirus; 5 Infectious hematopoietic hypodermal necrosis virus 6 Taura syndrome virus; 7 Baculovirus penaei; 8Polymerase chain reaction 9 Reverse transcription-PCR |
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TABLE 2. Viral diseases of marine fish |
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Fish Virus |
Available Diagnostic Method |
Limitation |
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| VNN1 | RT-PCRhistopathologyimmunofluorescence (IF) of enzyme linked immunosorbent assay (ELISA)cell culture method using SSN2-1 or GF3-1 | availability of antibody and fluorescence microscopesupply of cell lines | |
| Iridovirus | IF with monoclonal antibody (MAb) (tissue imprinting)PCR | availability of positive control of MAb and fluorescence microscope | |
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1Viral nervous necrosis; 2Striped snakehead; 3Grouper fin |
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4 December |
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08:30-09:00 |
Registration | ||
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09:00-19:20 |
Opening ceremony | ||
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09:30-10:10 |
D.V. Lightner (University of Arizona, USA) Advances in diagnosis and management of shrimp virus diseases in the Americas |
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10:10-10:30 |
Coffee break | ||
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10:30-11:10 |
T.W. Flegel (Mahidol University, Thailand) An overview of PCR techniques for shrimp disease diagnosis in Asia, with emphasis on Thailand |
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11:10-11:50 |
K. Mushiake (Japan Sea farming Association, Japan) Diagnostic and preventive practices for WSSV in Japan |
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11:50-13:30 |
Lunch break | ||
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13:30-14:00 |
L.D. de la Pena (SEAFDEC/AQD, Philippines) Progress and current status of diagnostic techniques for shrimp viral diseases at the SEAFDEC Aquaculture Department |
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14:00-14:40 |
K. Nakajima (National Research Institute of Aquaculture, Japan) Diagnostic and preventive practices for iridovirus in marine fish |
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14:40-15:20 |
T. Nakai (Hiroshima University, Japan) Diagnostic and preventive practices for viral nervous necrosis (VNN) |
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15:20-15:40 |
Coffee break | ||
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15:40-16:20 |
S. Kanchanakhan (Aquatic Animal Health Research Institute, Thailand) Diagnostic practices for marine fish viral diseases in Thailand |
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16:20-16:50 |
G.D. Lio-Po (SEAFDEC/AQD, Philippines) Progress and current status of diagnostic techniques for marine fish viral diseases at the SEAFDEC Aquaculture Department |
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5 December |
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08:30-10:30 |
Y. Inui (SEAFDEC/AQD, Philippines) Workshop: Considerations for training program in SEAFDEC |
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10:30-10:50 |
Coffee break |
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10:50-11:30 |
E. Ringo (Norwegian School of Veterinary Science, Norway) Probiotics in aquaculture |
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11:30-12:10 |
C.R. Lavilla-Pitogo (SEAFDEC/AQD, Philippines) Selection of probiotics for shrimp and crab hatcheries |
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12:10-13:30 |
Lunch break | ||
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13:30-14:10 |
R. Usero (Negros Prawn Producers Marketing Cooperative, Inc., Philippines) Integration of finfish in shrimp (Penaeus monodon) culture: an effective disease prevention strategy |
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14:10-14:40 |
G.D. Lio-Po (SEAFDEC/AQD, Philippines) Vibrio harveyi and the 'green water culture' of Penaeus monodon |
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14:40-15:00 |
Coffee break | ||
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15:00-15:30 |
Y. Inui (SEAFDEC/AQD, Philippines) Fish Disease Control Project of SEAFDEC Aquaculture Department |
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15:30-16:00 |
B. Hill (Centre for Environment, Fisheries & Aquaculture Sciences, UK) Global aquatic animal disease control activities of OIE Fish Diseases Commission |
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16:00-16:30 |
M.G. Bondad-Reantaso (Network of Aquaculture Centres in Asia-Pacific, Thailand) Recent Asian initiatives under NACA Regional Programme on aquatic animal health management |
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16:30-17:00 |
Discussion | ||
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17:00-17:30 |
Closing ceremony | ||
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6 December |
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| 08:00-11:00 | Tour of SEAFDEC/AQD facilities | ||